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human recombinant egf  (MedChemExpress)


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    Structured Review

    MedChemExpress human recombinant egf
    Human Recombinant Egf, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+egf/EGF%2C+Human/pm42251414-78-11-14
    Average 97 stars, based on 45 article reviews
    human recombinant egf - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Purification:

    Article Title: Mesenchymal stem cell-derived apoptotic extracellular vesicles loaded with Prussian blue nanoparticles attenuate severe acute pancreatitis via neutrophil extracellular traps resolution and acinar-ductal metaplasia promotion.
    Article Snippet: .. Purified acinar cells were resuspended in Waymouth’s medium (GibcoTM, 11220035, USA) supplemented with 2.5% FBS (Vivacell, C04001-500, China), 1% penicillinstreptomycin (GibcoTM, 15140-122, USA), 0.25 mg/mL trypsin inhibitor (Solarbio, T8031, China), and 25 ng/mL recombinant human EGF (MedChemExpress, HY-P7067, USA). .. Cell suspension was mixed with Matrigel (Corning, 354234, USA) (1:2 ratio), incubated at room temperature for 10 min, then 40 μL aliquots were plated in 24-well plates.

    Article Title: Mesenchymal stem cell-derived apoptotic extracellular vesicles loaded with Prussian blue nanoparticles attenuate severe acute pancreatitis via neutrophil extracellular traps resolution and acinar-ductal metaplasia promotion
    Article Snippet: .. Purified acinar cells were resuspended in Waymouth’s medium (GibcoTM, 11220035, USA) supplemented with 2.5% FBS (Vivacell, C04001-500, China), 1% penicillin-streptomycin (GibcoTM, 15140-122, USA), 0.25 mg/mL trypsin inhibitor (Solarbio, T8031, China), and 25 ng/mL recombinant human EGF (MedChemExpress, HY-P7067, USA). .. Purified acinar cells were resuspended in Waymouth’s medium (Gibco, 11220035, USA) supplemented with 2.5% FBS (Vivacell, C04001-500, China), 1% penicillin-streptomycin (Gibco, 15140-122, USA), 0.25 mg/mL trypsin inhibitor (Solarbio, T8031, China), and 25 ng/mL recombinant human EGF (MedChemExpress, HY-P7067, USA).

    Recombinant:

    Article Title: Mesenchymal stem cell-derived apoptotic extracellular vesicles loaded with Prussian blue nanoparticles attenuate severe acute pancreatitis via neutrophil extracellular traps resolution and acinar-ductal metaplasia promotion.
    Article Snippet: .. Purified acinar cells were resuspended in Waymouth’s medium (GibcoTM, 11220035, USA) supplemented with 2.5% FBS (Vivacell, C04001-500, China), 1% penicillinstreptomycin (GibcoTM, 15140-122, USA), 0.25 mg/mL trypsin inhibitor (Solarbio, T8031, China), and 25 ng/mL recombinant human EGF (MedChemExpress, HY-P7067, USA). .. Cell suspension was mixed with Matrigel (Corning, 354234, USA) (1:2 ratio), incubated at room temperature for 10 min, then 40 μL aliquots were plated in 24-well plates.

    Article Title: Insights into the pro-angiogenic effect of hydroxysafflor yellow A (HSYA): targeting HIF-1α and MMP9 in HMEC-1
    Article Snippet: .. HMEC-1 cells were commercially obtaind from Immocell (Xiamen, China), and were cultured in MCDB131 basal medium (Sigma-Aldrich, St. Louis, United States) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific, Waltham, United States), 1% penicillin-streptomycin (Thermo Fisher Scientific, Waltham, United States), 10 ng/mL recombinant human EGF (MedChemExpress, Monmouth Junction, United States), and 1 μg/mL hydrocortisone (SPExBIO, Houston, United States). ..

    Article Title: Perturbation of mammary epithelial cell apicobasal polarity by RHBDF1-facilitated nuclear translocation of PKCζ
    Article Snippet: MCF10A cells were purchased from American Type Culture Collection (ATCC, Rockefeller, USA), and cultured in the culture media with 5% CO 2 at 37 °C. .. The culture media formulation is as follows: 500 mL DMEM/F12 (Invitrogen, Carlsbad, USA), containing 5% (v/v) Horse serum (Solarbio, Beijing, China), 20 ng/mL of Recombinant Human EGF (PeproTech, Rocky Hill, USA), 0.5 μg/mL, 100 ng/mL and 10 μg/mL of Hydrocortisone, Cholera toxin and Insulin, respectively (MedChemExpress, New Jersey, USA), and 1% penicillin (50 U/mL)-streptomycin (50 μg/mL) (Invitrogen, Carlsbad, USA). .. The lentivirus: pLV[Exp]-EGFP: T2A: Puro-Null, pLV[Exp]-EGFP: T2A: Puro-EF1A > HA/hRHBDF1 and pLV[TetOn]-EGFP: T2A: Puro-TRE > HA/hRHBDF1 used to infect MCF10A cells were purchased from VectorBuilder (Guangzhou, China).

    Article Title: Perturbation of mammary epithelial cell apicobasal polarity by RHBDF1-facilitated nuclear translocation of PKCζ.
    Article Snippet: Cell culture and establishment of stable overexpressed RHBDF1 MCF-10 A cell lines MCF10A cells were purchased from American Type Culture Collection (ATCC, Rockefeller, USA), and cultured in the culture media with 5% CO2 at 37 °C. .. The culture media formulation is as follows: 500 mL DMEM/F12 (Invitrogen, Carlsbad, USA), containing 5% (v/v) Horse serum (Solarbio, Beijing, China), 20 ng/mL of Recombinant Human EGF (PeproTech, Rocky Hill, USA), 0.5 μg/ mL, 100 ng/mL and 10 μg/mL of Hydrocortisone, Cholera toxin and Insulin, respectively (MedChemExpress, New Jersey, USA), and 1% penicillin (50 U/mL)-streptomycin (50 μg/mL) (Invitrogen, Carlsbad, USA). .. The lentivirus: pLV[Exp]-EGFP: T2A: Puro-Null, pLV[Exp]-EGFP: T2A: Puro-EF1A > HA/hRHBDF1 and pLV[TetOn]-EGFP: T2A: Puro-TRE > HA/hRHBDF1 used to infect MCF10A cells were purchased from VectorBuilder (Guangzhou, China).

    Article Title: LDOC1 connects histone H2B monoubiquitination to tumor cell plasticity in non-small cell lung cancer.
    Article Snippet: Antibodies for flow cytometry included APC anti-human CD324 (E-Cadherin) (BioLegend, #324107), PE anti-vimentin (BioLegend, #677804), and isotype controls (BioLegend, #401209, #400121, #400113, #400507). .. Chemical reagents included recombinant human TGF-β (MCE, HY-P7118), the proteasome inhibitor bortezomib (MCE, HY-10227) and recombinant human EGF (MCE, HY-P7109). ..

    Article Title: LDOC1 connects histone H2B monoubiquitination to tumor cell plasticity in non-small cell lung cancer
    Article Snippet: Antibodies for flow cytometry included APC anti-human CD324 (E-Cadherin) (BioLegend, #324107), PE anti-vimentin (BioLegend, #677804), and isotype controls (BioLegend, #401209, #400121, #400113, #400507). .. Chemical reagents included recombinant human TGF-β (MCE, HY-P7118), the proteasome inhibitor bortezomib (MCE, HY-10227) and recombinant human EGF (MCE, HY-P7109). ..

    Article Title: Mesenchymal stem cell-derived apoptotic extracellular vesicles loaded with Prussian blue nanoparticles attenuate severe acute pancreatitis via neutrophil extracellular traps resolution and acinar-ductal metaplasia promotion
    Article Snippet: .. Purified acinar cells were resuspended in Waymouth’s medium (GibcoTM, 11220035, USA) supplemented with 2.5% FBS (Vivacell, C04001-500, China), 1% penicillin-streptomycin (GibcoTM, 15140-122, USA), 0.25 mg/mL trypsin inhibitor (Solarbio, T8031, China), and 25 ng/mL recombinant human EGF (MedChemExpress, HY-P7067, USA). .. Purified acinar cells were resuspended in Waymouth’s medium (Gibco, 11220035, USA) supplemented with 2.5% FBS (Vivacell, C04001-500, China), 1% penicillin-streptomycin (Gibco, 15140-122, USA), 0.25 mg/mL trypsin inhibitor (Solarbio, T8031, China), and 25 ng/mL recombinant human EGF (MedChemExpress, HY-P7067, USA).

    Article Title: A Fluorescent Probe Targeting EGFR for the Stratification of Prostate Cancer Cells.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Cell Culture:

    Article Title: Insights into the pro-angiogenic effect of hydroxysafflor yellow A (HSYA): targeting HIF-1α and MMP9 in HMEC-1
    Article Snippet: .. HMEC-1 cells were commercially obtaind from Immocell (Xiamen, China), and were cultured in MCDB131 basal medium (Sigma-Aldrich, St. Louis, United States) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific, Waltham, United States), 1% penicillin-streptomycin (Thermo Fisher Scientific, Waltham, United States), 10 ng/mL recombinant human EGF (MedChemExpress, Monmouth Junction, United States), and 1 μg/mL hydrocortisone (SPExBIO, Houston, United States). ..

    Formulation:

    Article Title: Perturbation of mammary epithelial cell apicobasal polarity by RHBDF1-facilitated nuclear translocation of PKCζ
    Article Snippet: MCF10A cells were purchased from American Type Culture Collection (ATCC, Rockefeller, USA), and cultured in the culture media with 5% CO 2 at 37 °C. .. The culture media formulation is as follows: 500 mL DMEM/F12 (Invitrogen, Carlsbad, USA), containing 5% (v/v) Horse serum (Solarbio, Beijing, China), 20 ng/mL of Recombinant Human EGF (PeproTech, Rocky Hill, USA), 0.5 μg/mL, 100 ng/mL and 10 μg/mL of Hydrocortisone, Cholera toxin and Insulin, respectively (MedChemExpress, New Jersey, USA), and 1% penicillin (50 U/mL)-streptomycin (50 μg/mL) (Invitrogen, Carlsbad, USA). .. The lentivirus: pLV[Exp]-EGFP: T2A: Puro-Null, pLV[Exp]-EGFP: T2A: Puro-EF1A > HA/hRHBDF1 and pLV[TetOn]-EGFP: T2A: Puro-TRE > HA/hRHBDF1 used to infect MCF10A cells were purchased from VectorBuilder (Guangzhou, China).

    Article Title: Perturbation of mammary epithelial cell apicobasal polarity by RHBDF1-facilitated nuclear translocation of PKCζ.
    Article Snippet: Cell culture and establishment of stable overexpressed RHBDF1 MCF-10 A cell lines MCF10A cells were purchased from American Type Culture Collection (ATCC, Rockefeller, USA), and cultured in the culture media with 5% CO2 at 37 °C. .. The culture media formulation is as follows: 500 mL DMEM/F12 (Invitrogen, Carlsbad, USA), containing 5% (v/v) Horse serum (Solarbio, Beijing, China), 20 ng/mL of Recombinant Human EGF (PeproTech, Rocky Hill, USA), 0.5 μg/ mL, 100 ng/mL and 10 μg/mL of Hydrocortisone, Cholera toxin and Insulin, respectively (MedChemExpress, New Jersey, USA), and 1% penicillin (50 U/mL)-streptomycin (50 μg/mL) (Invitrogen, Carlsbad, USA). .. The lentivirus: pLV[Exp]-EGFP: T2A: Puro-Null, pLV[Exp]-EGFP: T2A: Puro-EF1A > HA/hRHBDF1 and pLV[TetOn]-EGFP: T2A: Puro-TRE > HA/hRHBDF1 used to infect MCF10A cells were purchased from VectorBuilder (Guangzhou, China).



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